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Catalog Number Size Price
LS-C357632-100 100 µg (1 mg/ml) $476 
PD-L2 / PDCD1LG2 / CD273 Antibody - Flow cytometry of PD-L2 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human PD‑L2 / PDCD1LG2 / CD273 Antibody (clone ANC8D12, Biotin) LS‑C357632

Monoclonal Mouse anti‑Human PD‑L2 / PDCD1LG2 / CD273 Antibody (clone ANC8D12, Biotin) LS‑C357632

Antibody:
PD-L2 / PDCD1LG2 / CD273 Mouse anti-Human Monoclonal (Biotin) (ANC8D12) Antibody
Application:
Flo, ELISA, Block
Reactivity:
Human
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$476
LS-C357632-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PD-L2 / PDCD1LG2 / CD273 Mouse anti-Human Monoclonal (Biotin) (ANC8D12) Antibody
Application:
Flo, ELISA, Block
Reactivity:
Human
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
CD273 antibody LS-C357632 is a biotin-conjugated mouse monoclonal antibody to human CD273 (PD-L2 / PDCD1LG2). Validated for Block, ELISA and Flow.
Target
Human PD-L2 / PDCD1LG2 / CD273
Synonyms
PDCD1LG2 | B7 dendritic cell molecule | B7-DC | B7DC | BA574F11.2 | CD273 | Btdc | CD273 antigen | PD-L2 | PDCD1 ligand 2 | PD-1-ligand 2 | Programmed death ligand 2 | Butyrophilin B7-DC | PD-1 ligand 2 | PDCD1L2 | PDL2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
ANC8D12
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant human CD273(B7-DC).
Specificity
Antibody <span style="font-size: 11.0pt; mso-bidi-font-size: 10.0pt;">ANC8D12 binds to recombinant CD273 and cell surface CD273 expressed on monocyte derived Dendritic cells (moDC), and blocks binding of recombinant CD279 to CD273 in EIA and FACS.
Applications
  • Flow Cytometry
  • ELISA
  • Blocking
  • Applications tested for the base form of this product only
Usage
Human monocyte derived Dendritic cells were prepared in 8 day culture. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of antihuman CD273/Biotin at concentration of 10 ug/ml. They were then washed twice and incubated with Streptavidin/R-PE, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to isotype control Mouse IgG2a/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD273-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PD-L2 / PDCD1LG2 / CD273
Q9BQ51 NM_025239 NP_079515.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2021