Human PCNA

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein A purified

Unmodified

Rat PCNA made in the protein A vector of pR1T2T.

Recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells, proliferating cells in solid tumors, and blast cells in leukemia patients. Species cross-reactivity: Invertebrates and vertebrates.

• IHC
• IHC - Paraffin
• Immunofluorescence
• Western blot (1:250 - 1:1000)
• Immunoprecipitation
• Flow Cytometry

Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and Indirect Flow Cytometry. Western Blot: 1:250-1:1000, Nuclear extracts are preferred over whole cell protein extracts. Detects a polypeptide at 36kD with an isoelectric point of 4.8. Immunohistochemistry (paraffin embedded tissue sections only): 1:20-1:200, may be used on formalin (buffered or unbuffered), methacarn and Bouin's reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air drying overnight on poly-L-lysine coated slides is recommended. Incubate for 1 hour at 25?C using standard ABC. Positive control: Tonsil or reactive lymph node.

PBS, pH 7.2, 1 mg/ml BSA, 0.1% Sodium Azide

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P12004 NCBI: NM_002592 NP_002583.1