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| Catalog Number | Size | Price |
|---|---|---|
| LS-B9987-0.05 | 0.05 mg (1 mg/ml) | $460 |
1 of 2
2 of 2
PathPlus™ Monoclonal Mouse anti‑Rat PCNA Antibody (clone PC10, IHC, WB) LS‑B9987
PathPlus™ Monoclonal Mouse anti‑Rat PCNA Antibody (clone PC10, IHC, WB) LS‑B9987
Note: This antibody replaces LS-C43735
Antibody:
PCNA Mouse anti-Rat Monoclonal (PC10) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo
Reactivity:
Rat, Human, Mouse, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Atlantic salmon, Monkey-Cynomolgus, Monkey-Rhesus
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PCNA Mouse anti-Rat Monoclonal (PC10) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo
Reactivity:
Rat, Human, Mouse, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Atlantic salmon, Monkey-Cynomolgus, Monkey-Rhesus
Format:
Unconjugated, Unmodified
Specifications
Description
PCNA (Proliferating cell nuclear antigen) is a replication factor that plays critical roles in DNA replication and repair. It is a cofactor of DNA polymerase delta, and acts as a homotrimer to increase the processivity of leading strand synthesis during DNA replication. It is ubiquitinated in response to DNA damage, and involved in the RAD6-dependent DNA repair pathway. Antibodies to PCNA are used as a marker of cell proliferation. It is expressed in the nucleus of proliferating cell populations.
References: Enzymes 2016 39:231; Nature Reviews Mol Cell Biol 2014 14:269
Host
Mouse
Reactivity
Rat, Human, Mouse, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Atlantic salmon, Monkey-Cynomolgus, Monkey-Rhesus
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
PC10
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Rat PCNA made in the protein A expression vector pR1T2T.
Specificity
Recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kD nuclear protein vital for cellular DNA synthesis at the replication fork through its interaction with. PCNA is the auxilliary protein for DNA polymerase delta. PCNA is highly conserved between mammalian species and other vertebrates. Clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse, chicken and abalone.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Western blot (1:1000)
- Immunoprecipitation
- Flow Cytometry (1:100)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C for up to 2 weeks. Long term: aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PCNA
Validation
Anti-PCNA / Cyclin antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PCNA / Cyclin antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PCNA / Cyclin antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PCNA / Cyclin antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
PathPlus™ PCNA Antibody (clone PC10) for IHC, WB/Western, IP, Flow LS-B9987 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Invertebrate, Vertebrate
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Pig
Applications:
IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
