Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Locations
Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
Telephone Numbers
Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-B7878-50 | 50 µg (0.5 mg/ml) | $460 |
1 of 3
2 of 3
3 of 3
PathPlus™ Monoclonal Mouse anti‑Human PCNA Antibody (clone PC10 (a.k.a. 3F81), IHC, WB) LS‑B7878
PathPlus™ Monoclonal Mouse anti‑Human PCNA Antibody (clone PC10 (a.k.a. 3F81), IHC, WB) LS‑B7878
Note: This antibody replaces LS-C106804
Antibody:
PCNA Mouse anti-Human Monoclonal (PC10 (a.k.a. 3F81)) Antibody
Application:
IHC, IHC-P, WB, IP, Flo
Reactivity:
Human, Invertebrate, Vertebrate
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PCNA Mouse anti-Human Monoclonal (PC10 (a.k.a. 3F81)) Antibody
Application:
IHC, IHC-P, WB, IP, Flo
Reactivity:
Human, Invertebrate, Vertebrate
Format:
Unconjugated, Unmodified
Specifications
Description
PCNA (Proliferating cell nuclear antigen) is a replication factor that plays critical roles in DNA replication and repair. It is a cofactor of DNA polymerase delta, and acts as a homotrimer to increase the processivity of leading strand synthesis during DNA replication. It is ubiquitinated in response to DNA damage, and involved in the RAD6-dependent DNA repair pathway. Antibodies to PCNA are used as a marker of cell proliferation. It is expressed in the nucleus of proliferating cell populations.
References: Enzymes 2016 39:231; Nature Reviews Mol Cell Biol 2014 14:269
Host
Mouse
Reactivity
Human, Invertebrate, Vertebrate
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Clone
PC10 (a.k.a. 3F81)
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant rat PCNA.
Applications
- IHC
- IHC - Paraffin (20 µg/ml)
- Western blot
- Immunoprecipitation
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The PC10 (a. k. a. 3F81) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PCNA
Validation
Anti-PCNA / Cyclin antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-PCNA / Cyclin antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-PCNA / Cyclin antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-PCNA / Cyclin antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
See More About...
LSBio Ratings
PathPlus™ PCNA Antibody (clone PC10 (a.k.a. 3F81)) for IHC, WB/Western, IP, Flow LS-B7878 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Rat, Human, Mouse, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Atlantic salmon, Monkey-Cynomolgus, Monkey-Rhesus
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Pig
Applications:
IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
