Isochrysis galbana PCNA

Rabbit

Isochrysis galbana, Algae (tested or 100% immunogen sequence identity)

Algae (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 50-75 of Isochrysis galbana PCNA.

A BLAST analysis was used to suggest that this antibody would react with PCNA from the following alga sources: Prorocentrum minimum, Tetraselmis chui, Pleurochrysis carterae and Isochrysis galbana. Expect partial reactivity with PCNA from other alga sources as well as carrot, tobacco, peas, soybean and rice based on partial protein sequence homology.  Species that have been tested but yielded no positive results are Pfiesteria piscicida, Emiliania huxleyi, Akashiwo sanguinea, Rhodomonas sp., Karlodinium micrum, and Skletonema costatum. Reactivity with PCNA from other sources has not been determined.

• Western blot (1:5000 - 1:32000)
• Immunoprecipitation (1:100)
• ELISA (1:10000 - 1:50000)

This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at -29 kD in size corresponding to PCNA by western blotting in the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P12004 NCBI: NM_002592 NP_002583.1