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Catalog Number	Size	Price
LS-C745019-25	25 µl (1 mg/ml)	$304

PARP1 Antibody - Western Blot of recombinant PARP1 with Rabbit anti-PARP1 (internal) antibody. Lane 1: PARP1-autocatalytic domain recombinant protein. Load: 0.05 µg per lane. Primary antibody: PARP1 (internal) antibody at 1µg/mL for overnight at 4°C. Secondary antibody: HRP Gt-a-rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~19 kDa for rPARP1 (internal) Other band(s): none.

PARP1 Antibody - Western Blot of rabbit anti-PARP1 antibody. Lane 1: Molecular Weight ladder. Lane 2: OVCAR-8 Wild Type. Lane 3: PARP1-KO. Lane 4: PARP2-KO. Lane 5: PARP3-KO. Lane 6: PARP4-KO Lane 7: PARP5a-KO. Lane 8: PARP5b-KO. Lane 9: PARP6-KO. Lane 10: PARP7-KO. Lane 11: PARP8-KO. Lane 12: PARP9-KO. Lane 13: PARP10-KO. Lane 14: PARP12-KO. Lane 15: PARP13-KO. Lane 16: PARP14-KO. Lane 17: PARP16-KO. Load: 5.0 µg per lane. Primary antibody: PARP1 antibody at 1ug/mL overnight at 4°C. Secondary antibody: Goat anti-rabbit Peroxidase secondary antibody at 1:40,000 for 30 min at RT. Blocking Buffer: MB-073 for 30 min at RT. Predicted size: ~113kDa for PARP1. Observed nonspecific ~40kDa.

PARP1 Antibody - Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 Antibodies. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load 5 ? per lane. Primary Antibody: Blot A: Anti-PARP1- n term; Blot B: Anti-PARP1- internal at 1?/mL for overnight at 4?. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4?. Predicted/Observed size: 113 kDa for endogenous PARP1. Other band(s): nonspecific ~ 40kDa in PARP1-AD only.

PARP1 Antibody - Western Blot of rabbit Anti-PARP1 (internal) Antibody. Lane 1: Wild Type control lysates. Lane 2: Knock Out control lysates. Load: 5 µg per lane. Primary antibody: PARP1 (internal) antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~113kDa endogenous for PARP1. Other band(s): nonspecific ~ 40kDa.

PARP1 Antibody - Peggy Sue Size Separation Electropherogram of OVCAR-8 lysates in no-salt buffer and detected with Anti-PARP1 (internal). UV immobilization time: 250 seconds. Protein concentration: 577 µg/mL; 120 s UV immobilization. Primary antibody concentration: 20µg/mL. Primary antibody incubation time: 180 min. Exposure time: 10 seconds. Predicted/observed: ~116 kDa.

PARP1 Antibody - OVCAR-8 Wild Type Lysate separated on 2D SDS-PAGE and blotted on PVDF to analyze immunocoverage of PARP1 antibody specific for the autocatalytic domain of PARP1. Primary Antibody: Anti-PARP1 (internal) antibody 1:200 overnight at 4°C. Secondary Antibody: Goat anti-rabbit Peroxidase at 1:2,000 at RT for 30min. Blocking Buffer: BlockOut for 30min at RT. Predicted/observed: ~110 kDa and pI 9.7. Other spots detected: cleavage products of PARP1.

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Polyclonal Rabbit anti‑Human PARP1 Antibody (C‑Terminus, WB) LS‑C745019

Name: Anti-PARP1 Antibody | Rabbit anti-Human Polyclonal IE,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C745019-25
Price: 304.0000 USD
Availability: InStock

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Antibody:

PARP1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

WB, IE

Reactivity:

Human

Format:

Unconjugated, Unmodified

$304

LS-C745019-25

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PARP1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

WB, IE

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

PARP1 antibody LS-C745019 is an unconjugated rabbit polyclonal antibody to human PARP1 (C-Terminus). Validated for IE and WB.

Target

Human PARP1

Synonyms

See All PARP1 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity chromatography

Modifications

Unmodified

Immunogen

PARP1 (internal) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with c-terminus region of human PARP1 autocatalytic domain recombinant protein.

Epitope

C-Terminus

Specificity

This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined.

Applications

Western blot (1:1000)
Immunoelectrophoresis

Usage

Applications should be user optimized.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About PARP1

Uniprot: P09874 NCBI: NM_001618 NP_001609.2