DJ-1 antibody LS-C14860 is an unconjugated mouse monoclonal antibody to human DJ-1 (PARK7). Validated for ELISA, IF, IHC and WB.
Human PARK7 / DJ-1
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Paraffin (1:10 - 1:20)
- IHC - Frozen (1:10 - 1:20)
- Immunofluorescence (1:10 - 1:20)
- Western blot (1:10 - 1:20)
- ELISA (1:100 - 1:1000)
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PARK7 / DJ-1 antibody was raised against recombinant human DJ-1 corresponding to the N-terminal region of the protein.
Specific for the human DJ-1 protein. On Western blots, it identifies the target band at ~24kD. Reactivity has been confirmed with recombinant DJ-1 protein and PC3 cell lysates by Western blot and with formalin fixed, paraffin-embedded human cerebellum, astrocytoma, kidney, liver and prostate tissues by immunohistochemistry.
Suitable for use in Immunohistochemistry, Western Blot, Immunofluorescence and ELISA. Immunohistochemistry (frozen and paraffin): 5-10 ug/ml. Western Blot: 5-10 ug/ml. Immunofluorescence: 5-10 ug/ml. ELISA: 0.1-1 ug/ml. For best results in immunohistochemistry with formalin-fixed, paraffin-embedded tissues, pretreatment with trypsin is recommended prior to staining.
0.1% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.