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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Paraplegin / SPG7 Antibody (clone 3H10) LS‑C175082
SPG7 antibody LS-C175082 is an unconjugated mouse monoclonal antibody to human SPG7 (Paraplegin). Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C175082-100
100 µl (1 mg/ml)
$335

Popular Paraplegin / SPG7 Products

SPG7 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded Human Kidney tissue using anti-SPG7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Species: Human, Monkey, Mouse, Rat, Dog
Applications: Immunofluorescence, Western blot, Flow Cytometry
Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.
Species: Human, Mouse
Applications: Immunofluorescence, Western blot, Flow Cytometry

Product Description

SPG7 antibody LS-C175082 is an unconjugated mouse monoclonal antibody to human SPG7 (Paraplegin). Validated for Flow, IF and WB.
About Paraplegin / SPG7
Paraplegin / SPG7 is a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. Q9UQ90 NM_003119 NP_003110.1

SPG7 Antibody, Cell adhesion regulator Antibody, Cell matrix adhesion regulator Antibody, Paraplegin Antibody, PGN Antibody, SPG5C Antibody, Spastic paraplegia 7 protein Antibody, CAR Antibody, CMAR Antibody


Specifications

Target
Human Paraplegin / SPG7
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [3H10]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7 (NP_003110) produced in E. coli.
Specificity
Human Paraplegin / SPG7
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunofluorescence

Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-SPG7 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-SPG7 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunofluorescence

Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-SPG7 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-SPG7 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunofluorescence

Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-SPG7 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-SPG7 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunofluorescence

Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-SPG7 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-SPG7 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Requested From: United States
Date Requested: 9/25/2018

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