SHC antibody LS-C10121 is an unconjugated mouse monoclonal antibody to SHC (p66) from human, bovine, dog and other species. Validated for ELISA, IHC and WB.
Human, Monkey, Bovine, Dog, Horse, Pig, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
- Western blot (0.5 µg/ml)
- ELISA (0.05 µg/ml)
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p66 / SHC antibody was raised against synthetic peptide corresponding to aa 314-320 (DPSpYVNV) of human shc phosphorylated at Tyr317 (KLH).
Recognizes human shc phosphorylated at Tyr317. Species cross-reactivity: mouse and canine.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 0.5 ug/ml for HRPO/ECL detection. Use Casein/Tween-20 based blocking and Blot incubation buffers. ELISA: 0.05 ug/ml. Positive control: H2030-03H1: HepG2 Cell Lysate, Vanadate Treated.
PBS, 0.09% Sodium Azide, Sucrose, PEG
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.