SHC antibody LS-C10117 is an unconjugated rabbit polyclonal antibody to human SHC (p66). Validated for WB.
(tested or 100% immunogen sequence identity)
- Western blot (0.1 - 1 µg/ml)
p66 / SHC antibody was raised against synthetic phosphopeptide derived from the region of human Shc that contains tyrosines 239 and 240. The sequence is conserved in mouse.
Reactivity: Human Shc. Mouse Shc has not been tested. This antibody cross-reacts with insulin receptor in CHO cells over-expressing insulin receptor.
Suitable for use in Western blotting applications. Western Blot: 0.1-1.0 ug/ml. At 0.50 ug/ml, the dilution provides 100 ml working solution, which at 10 ml/blot allows 10 blots to be performed. Positive control: A431 cells +/- EGF.
PBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.2, 0.1% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.