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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C25959-50 50 µl $415 
Antibody

Polyclonal Rabbit anti‑Rat P2RX3 / P2X3 Antibody (aa383‑397, IHC, IF, WB) LS‑C25959

Polyclonal Rabbit anti‑Rat P2RX3 / P2X3 Antibody (aa383‑397, IHC, IF, WB) LS‑C25959

Note: This antibody replaces LS-C91221, LS-C91222, LS-C91220, LS-C25958, LS-C91219
Antibody:
Rabbit Polyclonal to Rat P2RX3 / P2X3
Application:
IHC, ICC, IF, WB
Reactivity:
Rat, Mouse, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$415 
LS-C25959-50
50 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Rat P2RX3 / P2X3
Application:
IHC, ICC, IF, WB
Reactivity:
Rat, Mouse, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
P2X3 antibody LS-C25959 is an unconjugated rabbit polyclonal antibody to P2X3 (P2RX3) (aa383-397) from rat. It is reactive with mouse, rat and hamster. Validated for ICC, IF, IHC and WB.
Target
Rat P2RX3 / P2X3
Synonyms
P2RX3 | ATP receptor | p2X3 | p2X3 purinoceptor | p2X purinoceptor 3 | Purinergic receptor P2X3 | Purinoceptor P2X3 | Purinergic receptor | p2RX3 | p2X receptor 3 | p2X receptor, subunit 3
Host
Rabbit
Reactivity
Rat, Mouse, Hamster (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • ICC (1:500)
  • Immunofluorescence
  • Western blot (1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide corresponding to aa383-397 (VEKQSTDSGAYSIGH) of rat P2X3. Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%); Elephant, Panda, Bovine, Dog, Horse (93%); Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Rabbit, Opossum (87%).
Epitope
aa383-397
Specificity
Recognizes rat P2X3 Receptor. Species cross-reactivity: Human and primate.
Usage
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Membranes were rinsed with PBS and incubated with Goat anti-rat IgG labeled with HRP for 1 hour at RT. Blocking buffer was removed. Slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times with PBS. Slides were incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times with PBS and coverslipped. Staining was examined using Fluorescence Microscopy. Immunocytochemistry: 1:500. P2X3 transfected cells were processed for Indirect Immunofluorescence. Media was removed. Cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for Indirect Immunofluorescence as described for Immunohistochemistry.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About P2RX3 / P2X3
P56373 NM_002559 NP_002550.2

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Requested From: United States
Date Requested: 7/5/2020