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Catalog Number Size Price
LS-C775700-100 100 µg (1 mg/ml) $528 
OGT / O-GLCNAC Antibody - Immunohistochemistry analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody  at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Citrulline Antibody (C) Composite.
OGT / O-GLCNAC Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
OGT / O-GLCNAC Antibody - Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
OGT / O-GLCNAC Antibody - Immunohistochemistry analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody  at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Citrulline Antibody (C) Composite.
OGT / O-GLCNAC Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
OGT / O-GLCNAC Antibody - Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
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Monoclonal Mouse anti‑All species OGT / O‑GLCNAC Antibody (clone 9H6, FITC, IHC, IF, WB) LS‑C775700

Monoclonal Mouse anti‑All species OGT / O‑GLCNAC Antibody (clone 9H6, FITC, IHC, IF, WB) LS‑C775700

Antibody:
OGT / O-GLCNAC Mouse Monoclonal (FITC) (9H6) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
All species
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$528
LS-C775700-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
OGT / O-GLCNAC Mouse Monoclonal (FITC) (9H6) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
All species
Format:
FITC, Unmodified
Other formats:

Specifications

Description
O-GLCNAC antibody LS-C775700 is an FITC-conjugated mouse monoclonal antibody to all species O-GLCNAC (OGT). Validated for ELISA, IF, IHC and WB.
Target
All species OGT / O-GLCNAC
Synonyms
OGT | HRNT1 | O-GLCNAC
Host
Mouse
Reactivity
All species
Clonality
IgM Monoclonal
Clone
9H6
Conjugations
FITC. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 594, Biotin, PerCP, RPE.
Purification
PEG purified
Modifications
Unmodified
Immunogen
Synthetic ß-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for B-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.
Applications
  • IHC (1:25)
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
  • ELISA (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About OGT / O-GLCNAC
O15294 U77413 AAB63466.1

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Requested From: United States
Date Requested: 4/19/2024