OAS1 antibody LS-C667654 is an unconjugated rabbit polyclonal antibody to human OAS1. Validated for ICC, IF, IHC and WB.
(tested or 100% immunogen sequence identity)
- IHC (1:50 - 1:200)
- IHC - Paraffin
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
OAS1 antibody was raised against recombinant full length protein of human OAS1
Recognizes endogenous levels of OAS1 protein.
Western blot analysis of OAS1 expression in mouse liver (A) whole cell lysates. Immunohistochemical analysis of OAS1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of OAS1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
For research use only.