Mouse NUMB

Rabbit

Mouse, Human (tested or 100% immunogen sequence identity)

Rat (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.

Immunoaffinity purified

Unmodified

A synthetic peptide made to a C-Terminus of the murine numb protein sequence (between residues 600-653). Percent identity by BLAST analysis: Rat (90%).

aa600-653

All four isoforms of the Numb protein.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (0.2 - 0.5 µg/ml)

Immunohistochemistry: LS-B265 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B265 was determined to be 2.5 ug/ml.

Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide

Short term: 4°C; Long term: -70°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P49757 NCBI: NM_003744 NP_003735.3