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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NUDT2 Antibody LS‑C482717
NUDT2 antibody LS-C482717 is an unconjugated rabbit polyclonal antibody to NUDT2 from human, mouse and rat. Validated for ICC, IF and WB.
Catalog
Size
Price
LS-C482717-50
50 µl (1 mg/ml)
$255
LS-C482717-100
100 µl (1 mg/ml)
$295
LS-C482717-200
200 µl (1 mg/ml)
$375
Description
NUDT2 antibody LS-C482717 is an unconjugated rabbit polyclonal antibody to NUDT2 from human, mouse and rat. Validated for ICC, IF and WB.
Target
Human NUDT2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
Immunogen
NUDT2 antibody was raised against recombinant full length Human NUDT2.
Specificity
Recognizes endogenous levels of NUDT2 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About NUDT2
P50583 NM_001161 NP_001152.1

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Images

Immunofluorescence

Immunofluorescent analysis of NUDT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of NUDT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of NUDT2 expression in SKOV3 (A); HL60 (B); mouse kidney (C); mouse brain (D); rat heart (E) whole cell lysates.
Western blot analysis of NUDT2 expression in SKOV3 (A); HL60 (B); mouse kidney (C); mouse brain (D); rat heart (E) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of NUDT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of NUDT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of NUDT2 expression in SKOV3 (A); HL60 (B); mouse kidney (C); mouse brain (D); rat heart (E) whole cell lysates.
Western blot analysis of NUDT2 expression in SKOV3 (A); HL60 (B); mouse kidney (C); mouse brain (D); rat heart (E) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of NUDT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of NUDT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of NUDT2 expression in SKOV3 (A); HL60 (B); mouse kidney (C); mouse brain (D); rat heart (E) whole cell lysates.
Western blot analysis of NUDT2 expression in SKOV3 (A); HL60 (B); mouse kidney (C); mouse brain (D); rat heart (E) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of NUDT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunofluorescent analysis of NUDT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot

Western blot analysis of NUDT2 expression in SKOV3 (A); HL60 (B); mouse kidney (C); mouse brain (D); rat heart (E) whole cell lysates.
Western blot analysis of NUDT2 expression in SKOV3 (A); HL60 (B); mouse kidney (C); mouse brain (D); rat heart (E) whole cell lysates.

Requested From: United States
Date Requested: 12/11/2018
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