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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3897-50 | 50 µl | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human NT5E / eNT / CD73 Antibody (clone 1D7, IHC) LS‑B3897
IHC‑plus™ Monoclonal Mouse anti‑Human NT5E / eNT / CD73 Antibody (clone 1D7, IHC) LS‑B3897
Note: This antibody replaces LS-C108849
Antibody:
NT5E / eNT / CD73 Mouse anti-Human Monoclonal (1D7) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NT5E / eNT / CD73 Mouse anti-Human Monoclonal (1D7) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD73 antibody LS-B3897 is an unconjugated mouse monoclonal antibody to human CD73 (NT5E / eNT). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human NT5E / eNT / CD73
Synonyms
NT5E | 5-nucleotidase, ecto (CD73) | 5'-nucleotidase | 5 nucleotidase (CD73) | 5-NT | 5-nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 | CD73 antigen | E5NT | Ecto-5-nucleotidase | ENT | EN | NT | Purine 5-Prime-Nucleotidase | NT5 | NTE
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
1D7
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of NT5E expressed in E. coli.
Specificity
Human
Applications
- IHC
- IHC - Paraffin (1:200 - 1:400)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3897 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3897 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NT5E / eNT / CD73
Validation
Anti-NT5E / CD73 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-NT5E / CD73 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-NT5E / CD73 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-NT5E / CD73 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-NT5E / CD73 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-NT5E / CD73 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ NT5E / eNT / CD73 Antibody (clone 1D7) for IHC, ELISA LS-B3897 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA, Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse
Applications:
Western blot, Flow Cytometry, ELISA
Reactivity:
Human
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
