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Catalog Number Size Price
LS-C143060-500 500 µl $335 
Antibody

Nsr1p Antibody (clone 31C4) LS‑C143060

Nsr1p Antibody (clone 31C4) LS‑C143060

Antibody:
Mouse Monoclonal to Yeast Nsr1p
Application:
IF, WB
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C143060-500
500 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Yeast Nsr1p
Application:
IF, WB
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
Nsr1p antibody LS-C143060 is an unconjugated mouse monoclonal antibody to yeast Nsr1p. Validated for IF and WB.
Target
Yeast Nsr1p
Host
Mouse
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Clone
31C4
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • Immunofluorescence (1:500 - 1:5000)
  • Western blot (1:10000)
Immunogen
Nsr1p is localized in the nucleolus and binds to telomeric single-stranded oligonucleotide repeat sequence of the type and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport
Specificity
31C4 was isolated during a screen for monoclonal antibodies reactive with yeast nucleolar proteins. We determined the specificity by screening a yeast genomic library expressed as b-galactosidase fusion proteins in lgt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones b-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with EFEH, corresponding to amino acids 190 and onwards in Nsr1p. This peptide is ~20 amino acids into the first RRM domain. Thus, MCA-31C4 reacts with an epitope in the C-terminal 55% of Nsr1p. The antibody is provided as sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The IgG isotype and exact concentration are not known.
Presentation
Tissue Culture Supernatant, 10 mM Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/19/2019