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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NSMAF Antibody (aa262‑570) LS‑C185559
NSMAF antibody LS-C185559 is an unconjugated rabbit polyclonal antibody to human NSMAF. Validated for IHC and WB.
Catalog
Size
Price
LS-C185559-100
100 µl (0.93 mg/ml)
$335
Description
NSMAF antibody LS-C185559 is an unconjugated rabbit polyclonal antibody to human NSMAF. Validated for IHC and WB.
Target
Human NSMAF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NSMAF antibody was raised against recombinant fragment corresponding to a region within amino acids 262 and 570 of FAN (SwissProt Q92636). Percent identity by BLAST analysis: Human (100%); Mouse (93%); Rat (91%).
Epitope
aa262-570
Specificity
Human NSMAF
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About NSMAF
Q92636 NM_003580 NP_003571.2

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Images

Immunohistochemistry

IHC of paraffin-embedded T (gastric CA) using NSMAF antibody at 1:100 dilution.
IHC of paraffin-embedded T (gastric CA) using NSMAF antibody at 1:100 dilution.

Western blot

Western blot of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate., B: NSMAF transfected lysate. 7.5% SDS PAGE. NSMAF antibody diluted at 1:500
Western blot of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate., B: NSMAF transfected lysate. 7.5% SDS PAGE. NSMAF antibody diluted at 1:500

Immunohistochemistry

IHC of paraffin-embedded T (gastric CA) using NSMAF antibody at 1:100 dilution.
IHC of paraffin-embedded T (gastric CA) using NSMAF antibody at 1:100 dilution.

Western blot

Western blot of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate., B: NSMAF transfected lysate. 7.5% SDS PAGE. NSMAF antibody diluted at 1:500
Western blot of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate., B: NSMAF transfected lysate. 7.5% SDS PAGE. NSMAF antibody diluted at 1:500

Immunohistochemistry

IHC of paraffin-embedded T (gastric CA) using NSMAF antibody at 1:100 dilution.
IHC of paraffin-embedded T (gastric CA) using NSMAF antibody at 1:100 dilution.

Western blot

Western blot of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate., B: NSMAF transfected lysate. 7.5% SDS PAGE. NSMAF antibody diluted at 1:500
Western blot of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate., B: NSMAF transfected lysate. 7.5% SDS PAGE. NSMAF antibody diluted at 1:500

Immunohistochemistry

IHC of paraffin-embedded T (gastric CA) using NSMAF antibody at 1:100 dilution.
IHC of paraffin-embedded T (gastric CA) using NSMAF antibody at 1:100 dilution.

Western blot

Western blot of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate., B: NSMAF transfected lysate. 7.5% SDS PAGE. NSMAF antibody diluted at 1:500
Western blot of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate., B: NSMAF transfected lysate. 7.5% SDS PAGE. NSMAF antibody diluted at 1:500

Requested From: United States
Date Requested: 11/19/2018
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