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Catalog Number Size Price
LS-C742385-100 100 µg (0.5 mg/ml) $503 
NRSF / REST Antibody - Antibody (0.3µg/ml) staining of HEK293 (A) and HeLa (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Polyclonal Goat anti‑Human NRSF / REST Antibody (Internal Region, WB) LS‑C742385

Polyclonal Goat anti‑Human NRSF / REST Antibody (Internal Region, WB) LS‑C742385

Antibody:
NRSF / REST Goat anti-Human Polyclonal (Internal Region) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C742385-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NRSF / REST Goat anti-Human Polyclonal (Internal Region) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
REST antibody LS-C742385 is an unconjugated goat polyclonal antibody to human REST (NRSF) (Internal Region). Validated for Peptide-ELISA and WB.
Target
Human NRSF / REST
Synonyms
REST | X2 box repressor | XBR | NRSF
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-PETPGAEDKGKSSK, from the internal region of the protein sequence according to NP_005603.3.
Epitope
Internal Region
Specificity
Reported variants represent identical protein: NP_001180437.1, NP_005603.3
Applications
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 140kDa band observed in nuclear lysates of cell lines HEK293 and HeLa (calculated MW of 122kDa according to NP_005603.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NRSF / REST
Q13127 NM_005612 NP_005603.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024