REST antibody LS-C17676 is an unconjugated rabbit polyclonal antibody to REST (NRSF) from human, mouse and rat. Validated for WB.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
NRSF / REST antibody was raised against gST fusion protein corresponding to residues 801-1097 of human REST (repressor element RE-1 silencing transcription factor). Also known as NRSF (neuronal restricted silencing factor).
Recognizes REST, Mr 120-200kD. Species cross-reactivity: Human, mouse and rat.
Suitable for use in Western Blot. Western Blot Analysis: 0.5-2 ug/ml of this lot detected REST in nuclear extracts from HeLa and Raji cells. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-REST (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates REST.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.