Mouse NRF1 / NRF-1

Rabbit

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse NRF1 protein corresponding to aa 1- 534 of the native protein.

• IHC
• IHC - Paraffin (2 - 5 µg/ml)
• Western blot (1:500 - 1:2000)
• ELISA (1:3000 - 1:10000)

Immunohistochemistry: LS-B43 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B43 was determined to be 2-5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q16656 NCBI: NM_005011 NP_005002.3