Target
Rat NR3C1/Glucocorticoid Receptor
Synonyms
NR3C1 | Glucocorticoid Receptor beta | GR | GCCR | GCR | Glucocorticoid receptor | Glucocorticoid receptor alpha | GRL
Reactivity
Rat, Mouse, Hamster
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Native glucocorticoid receptor purified from rat liver.
Specificity
Recognizes glucocorticoid receptor, Mr 85-100kD. Species cross-reactivity: Rat, mouse and hamster. Does not react with human.
Applications
- Western blot
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot Analysis: 1-8 ug/ml of this lot detected Glucocorticoid Receptor in RIPA lysates from L6, PC12, and 3T3/A31 cells. L6 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Glucocorticoid Receptor (4 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates glucocorticoid receptor (~97kD). Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate glucocorticoid receptor from WEHI-7 and WCL2 cells.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NR3C1/Glucocorticoid Receptor