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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C355579-200 200 µg (1 mg/ml) $445 
NR1H3 / LXR Alpha Antibody - IF using LXR alpha Antibody
NR1H3 / LXR Alpha Antibody - IF using LXR alpha Antibody
NR1H3 / LXR Alpha Antibody - Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of MCF7 (Lane 1), THP-1 (Lane 2), A549 (Lane 3), SH-SY5Y (Lane 4), HL-60 (Lane 5) and KARPAS 299 (Lane 6). The blot was probed with Rabbit Anti-LXR alpha Polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A 50 kDa band corresponding to LXR alpha was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using 12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk.
NR1H3 / LXR Alpha Antibody - IF using LXR alpha Antibody
NR1H3 / LXR Alpha Antibody - IF using LXR alpha Antibody
NR1H3 / LXR Alpha Antibody - Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of MCF7 (Lane 1), THP-1 (Lane 2), A549 (Lane 3), SH-SY5Y (Lane 4), HL-60 (Lane 5) and KARPAS 299 (Lane 6). The blot was probed with Rabbit Anti-LXR alpha Polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A 50 kDa band corresponding to LXR alpha was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using 12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk.
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Polyclonal NR1H3 / LXR Alpha Antibody (IF, WB) LS‑C355579

Polyclonal NR1H3 / LXR Alpha Antibody (IF, WB) LS‑C355579

Antibody:
Rabbit Polyclonal to Human NR1H3 / LXR Alpha
Application:
ICC, IF, WB
Reactivity:
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Dog, Goat, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$445 
LS-C355579-200
200 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human NR1H3 / LXR Alpha
Application:
ICC, IF, WB
Reactivity:
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Dog, Goat, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
LXR Alpha antibody LS-C355579 is an unconjugated rabbit polyclonal antibody to LXR Alpha (NR1H3) from human, mouse, rat and other species. Validated for ICC, IF and WB.
Target
Human NR1H3 / LXR Alpha
Synonyms
NR1H3 | Liver X receptor alpha | LXRA | LXR-a | Oxysterols receptor LXR-alpha | LXR alpha | Lxralpha | RLD-1
Host
Rabbit
Reactivity
Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Dog, Goat, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:50 - 1:500)
  • Immunofluorescence (1:50 - 1:500)
  • Western blot (1:300)
Immunogen
Synthetic peptide corresponding to aa318-329 (DFSYNREDFAKA) of human LXR alpha. Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Dog, Bat, Goat, Pig (100%); Trout, Pufferfish (92%); Zebrafish (83%).
Specificity
The antibody detects recombinant human liver X receptor (LXR) alpha. This antibody does not detect human recombinant LXR beta protein. It has been successfully used in ICC/IF and Western blot procedures. By Western blot, this antibody detects an ~64 kD protein representing recombinant human LXR alpha.
Presentation
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NR1H3 / LXR Alpha
Q13133 NM_005693 NP_005684.2

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Requested From: United States
Date Requested: 4/3/2020