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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2930-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human NPM1 / NPM / Nucleophosmin Antibody (clone 5E3, aa81‑294, IHC, WB) LS‑B2930
IHC‑plus™ Monoclonal Mouse anti‑Human NPM1 / NPM / Nucleophosmin Antibody (clone 5E3, aa81‑294, IHC, WB) LS‑B2930
Note: This antibody replaces LS-C93771
Antibody:
NPM1 / NPM / Nucleophosmin Mouse anti-Human Monoclonal (aa81-294) (5E3) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NPM1 / NPM / Nucleophosmin Mouse anti-Human Monoclonal (aa81-294) (5E3) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Nucleophosmin antibody LS-B2930 is an unconjugated mouse monoclonal antibody to human Nucleophosmin (NPM1 / NPM) (aa81-294). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NPM1 / NPM / Nucleophosmin
Synonyms
NPM1 | NPM | Nucleolar phosphoprotein B23 | Nucleolar protein NO38 | Numatrin | B23 | Nucleophosmin
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Clone
5E3
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human NPM (81-294aa) purified from E. coli
Epitope
aa81-294
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2930 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2930 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NPM1 / NPM / Nucleophosmin
Validation
Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NPM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NPM1 / NPM / Nucleophosmin Antibody (aa81-294, clone 5E3) for IHC, WB/Western, ELISA LS-B2930 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
![NPM1 / NPM / Nucleophosmin Antibody - Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/npm1-npm-nucleophosmin-antibody-clone-3b2-ls-c133351/51336_1027566.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications:
IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Xenopus, Chicken
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat, Bovine, Rabbit
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Reactivity:
Human
Range:
0.313-20 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
