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Antibodies > NOX4 > LS-C313066
 
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Catalog Number Size Price
LS-C313066-10 10 µg $318 
LS-C313066-100 100 µg $470 
NOX4 Antibody - NOX4 antibody. IHC(P): Rat Kidney Tissue.
NOX4 Antibody - Western blot analysis of NADPH oxidase 4/NOX4 using anti-NADPH oxidase 4/NOX4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human HK-2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SW579 whole cell lysates, Lane 7: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4/NOX4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NADPH OXIDASE 4/NOX4 at approximately 67KD. The expected band size for NADPH OXIDASE 4/NOX4 is at 67KD.
NOX4 Antibody - WB of NOX4 antibody. Lane 1: Rat Kidney Tissue Lysate. Lane 2: Rat Cardiac Muscle Tissue Lysate. Lane 3: Rat Spleen Tissue Lysate. Lane 4: HELA Cell Lysate. Lane 5: 293T Cell Lysate. Lane 6: MCF-7 Cell Lysate. Lane 7: SMMC Cell Lysate.
NOX4 Antibody - Flow Cytometry analysis of U20S cells using anti-NADPH oxidase 4/NOX4 antibody. Overlay histogram showing U20S cells stained with anti-NADPH oxidase 4/NOX4 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NADPH oxidase 4/NOX4 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
NOX4 Antibody - NOX4 antibody. IHC(P): Rat Kidney Tissue.
NOX4 Antibody - Western blot analysis of NADPH oxidase 4/NOX4 using anti-NADPH oxidase 4/NOX4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human HK-2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SW579 whole cell lysates, Lane 7: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4/NOX4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NADPH OXIDASE 4/NOX4 at approximately 67KD. The expected band size for NADPH OXIDASE 4/NOX4 is at 67KD.
NOX4 Antibody - WB of NOX4 antibody. Lane 1: Rat Kidney Tissue Lysate. Lane 2: Rat Cardiac Muscle Tissue Lysate. Lane 3: Rat Spleen Tissue Lysate. Lane 4: HELA Cell Lysate. Lane 5: 293T Cell Lysate. Lane 6: MCF-7 Cell Lysate. Lane 7: SMMC Cell Lysate.
NOX4 Antibody - Flow Cytometry analysis of U20S cells using anti-NADPH oxidase 4/NOX4 antibody. Overlay histogram showing U20S cells stained with anti-NADPH oxidase 4/NOX4 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NADPH oxidase 4/NOX4 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Mouse NOX4 Antibody (aa561‑578, IHC, WB) LS‑C313066

Polyclonal Rabbit anti‑Mouse NOX4 Antibody (aa561‑578, IHC, WB) LS‑C313066

(4.1) Rating
Submit Review (2) Publication(s) LSBio Guarantee
Note: This antibody replaces LS-C388976
Antibody:
NOX4 Rabbit anti-Mouse Polyclonal (aa561-578) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313066-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NOX4 Rabbit anti-Mouse Polyclonal (aa561-578) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NOX4 antibody LS-C313066 is an unconjugated rabbit polyclonal antibody to NOX4 (aa561-578) from mouse. It is reactive with human, mouse and rat. Validated for IHC and WB. Cited in 2 publications.
(2) Publication(s)
Target
Mouse NOX4
Synonyms
NOX4 | Kidney oxidase-1 | KOX | RENOX | KOX-1 | NADPH oxidase 4 | Renal NAD(P)H-oxidase
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of mouse NADPH oxidase 4(561-578 aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids.
Epitope
aa561-578
Specificity
EXpressed in brain, in all layers of the cerebellum, in pyramidal cells of the Ammon horn and in Purkinje cells (at protein level). Expressed in osteoclasts, leukocytes, kidney, liver and lung. .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOX4
Q9NPH5 NM_016931 NP_058627.1

LSBio Ratings

NOX4 Antibody (aa561-578) for IHC, WB/Western LS-C313066 has an LSBio Rating of
(4.1) Rating
Publications (4.1)

Publications (2)

Nuclear NADPH oxidase-4 associated with disease progression in renal cell carcinoma. Dharam Kaushik, Keith A Ashcraft, Hanzhang Wang, Karthigayan Shanmugasundaram, Pankil K Shah, Gabriela Gonzalez, Alia Nazarullah, Cooper B Tye, Michael A Liss, Deepak K Pruthi, Ahmed M Mansour, Wasim Chowdhury, Dean Bacich, Hao Zhang, Amanda L Watson, Karen Block, Denise O'Keefe, Ronald Rodriguez. Translational research : the journal of laboratory and clinical medicine. 2020 Sep;;223:44575.
Full Text Article PubMed: 32492552 PMC: PMC8111697
Effects of anagliptin on the stress induced accelerated senescence of human umbilical vein endothelial cells. Seon Mee Kang , Hye Sook Jung, Min Jeong Kwon, Soon Hee Lee, Jeong Hyun Park. Annals of Translational Medicine. 2021 May;;9:750.
Full Text Article PubMed: 34268363 PMC: PMC8246235
Submit a Publication for LS-C313066

Customer Reviews (0)

Submit a review for LS-C313066

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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