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Catalog Number Size Price
LS-C19109-200 200 µl (90 mg/ml) $511 
NOTCH1 Antibody - Immunohistochemistry of Rabbit anti-Notch1 antibody. Tissue: Exocrine glands of human pancreas. Fixation: FFPE. Primary antibody: Notch1 antibody at 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
NOTCH1 Antibody - Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot. Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods. A reaction with diluted primary antibody was followed by washing; reaction with a 1:10000 dilution of HRP conjugated Gt-a-Rabbit IgG, and color development. Lane M: Mol wt markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.
NOTCH1 Antibody - Immunohistochemistry of Rabbit anti-Notch1 antibody. Tissue: Exocrine glands of human pancreas. Fixation: FFPE. Primary antibody: Notch1 antibody at 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
NOTCH1 Antibody - Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot. Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods. A reaction with diluted primary antibody was followed by washing; reaction with a 1:10000 dilution of HRP conjugated Gt-a-Rabbit IgG, and color development. Lane M: Mol wt markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.
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Polyclonal Rabbit anti‑Human NOTCH1 Antibody (N‑Terminus, IHC, WB) LS‑C19109

Polyclonal Rabbit anti‑Human NOTCH1 Antibody (N‑Terminus, IHC, WB) LS‑C19109

Note: This antibody replaces LS-C73694
Antibody:
NOTCH1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$511
LS-C19109-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NOTCH1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NOTCH1 antibody LS-C19109 is an unconjugated rabbit polyclonal antibody to NOTCH1 (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Cited in 3 publications.
Target
Human NOTCH1
Synonyms
NOTCH1 | Notch 1 | TAN1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Epitope
N-Terminus
Specificity
This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Applications
  • IHC (1:200)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000 - 1:60000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody is reactive with Notch 1 by western blotting. A strong response was also detected by ELISA against the immunizing peptide. This product was assayed by immunoblot against recombinant mouse Notch constructs in 293 cells. An 80 kD band corresponding to Notch 1 was observed at a 1:500 dilution. This product was also assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG (H&L) [Goat] (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOTCH1
P46531 NM_017617 NP_060087.3

LSBio Ratings

NOTCH1 Antibody (N-Terminus) for IHC, WB/Western, ELISA LS-C19109 has an LSBio Rating of
Publications (4.2)

Publications (3)

Aberrant activation of notch signaling in human breast cancer. Stylianou S, Clarke RB, Brennan K. Cancer research. 2006 66:1517-25.

Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants. Solomon A, Mian Y, Ortega-Cava C, Liu VW, Gurumurthy CB, Naramura M, Band V, Band H. Developmental biology. 2008 316:191-9.

Effect of Notch activation on the regenerative response to acute renal failure. Gupta S, Li S, Abedin MJ, Wang L, Schneider E, Najafian B, Rosenberg M. American journal of physiology. Renal physiology. 2010 298:F209-15.

Customer Reviews (0)


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Requested From: United States
Date Requested: 8/15/2022