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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NMT1 Antibody (aa108‑157) LS‑C102217
NMT1 antibody LS-C102217 is an unconjugated rabbit polyclonal antibody to NMT1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C102217-50
50 µg
$365

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Applications: IHC, Western blot, ELISA

Product Description

NMT1 antibody LS-C102217 is an unconjugated rabbit polyclonal antibody to NMT1 from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Human NMT1
Synonyms
NMT1, Alternative, short form NMT-S, Long form, NMT-L, N-myristoyltransferase 1, NMT, NMT 1, Type I N-myristoyltransferase
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
NMT1 antibody was raised against synthetic peptide located between aa108-157 of human NMT1 (P30419, NP_066565). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Rabbit, Pig, Opossum, Turkey, Chicken (100%); Dog, Horse, Lizard, Xenopus (92%); Guinea pig, Zebrafish (85%).
Blocking Peptide
LS-E16884 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102217. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa108-157
Specificity
Human NMT1
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About NMT1
Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. P30419 NM_021079 NP_066565.1


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Images

Western blot

NMT1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NMT1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NMT1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NMT1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NMT1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NMT1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NMT1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NMT1 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/15/2018

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