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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C680951-50 50 µg $285 
LS-C680951-100 100 µg $385 
NLN / Neurolysin Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
NLN / Neurolysin Antibody - Immunofluorescence staining of HepG2 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
NLN / Neurolysin Antibody - Western Blot Positive WB detected in: 293 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate All lanes: NLN antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81 kDa Observed band size: 81 kDa
NLN / Neurolysin Antibody - Western Blot Positive WB detected in: 293 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate All Lanes: NLN antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81 KDa Observed band size: 81 KDa
NLN / Neurolysin Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
NLN / Neurolysin Antibody - Immunofluorescence staining of HepG2 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
NLN / Neurolysin Antibody - Western Blot Positive WB detected in: 293 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate All lanes: NLN antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81 kDa Observed band size: 81 kDa
NLN / Neurolysin Antibody - Western Blot Positive WB detected in: 293 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate All Lanes: NLN antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81 KDa Observed band size: 81 KDa
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Polyclonal Rabbit anti‑Human NLN / Neurolysin Antibody (IHC, IF, WB) LS‑C680951

Polyclonal Rabbit anti‑Human NLN / Neurolysin Antibody (IHC, IF, WB) LS‑C680951

Antibody:
Rabbit Polyclonal to Human NLN / Neurolysin
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$285 
LS-C680951-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human NLN / Neurolysin
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Neurolysin antibody LS-C680951 is an unconjugated rabbit polyclonal antibody to human Neurolysin (NLN). Validated for ELISA, IF, IHC and WB.
Target
Human NLN / Neurolysin
Synonyms
NLN | AGTBP | Angiotensin binding protein | Angiotensin-binding protein | Endopeptidase 24.16 | Endopeptidase 3.4.24.16 | Neurolysin | Neurolysin, mitochondrial | KIAA1226 | MEP | MOP | Neurotensin endopeptidase | EP24.16 | Microsomal endopeptidase | Mitochondrial oligopeptidase M
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant Human Neurolysin, mitochondrial protein (109-241AA)
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NLN / Neurolysin
Q9BYT8 NM_020726 NP_065777.1

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Requested From: United States
Date Requested: 7/6/2020