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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NIT1 Antibody (clone 4D12) LS-C115078

NIT1 antibody LS-C115078 is an unconjugated mouse monoclonal antibody to human NIT1. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C115078-100
100 µl (0.66 mg/ml)
$345
Description
NIT1 antibody LS-C115078 is an unconjugated mouse monoclonal antibody to human NIT1. Validated for Flow, IF, IHC and WB.
Target
Human NIT1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
4D12
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NIT1 antibody was raised against full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
Specificity
Human NIT1
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About NIT1
Q86X76 NM_005600 NP_005591.1

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Images

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody.
Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody.

Immunofluorescence

Anti-NIT1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1.
Anti-NIT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY NIT1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY NIT1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody.
Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody.

Immunofluorescence

Anti-NIT1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1.
Anti-NIT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY NIT1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY NIT1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody.
Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody.

Immunofluorescence

Anti-NIT1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1.
Anti-NIT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY NIT1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY NIT1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody.
Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody.

Immunofluorescence

Anti-NIT1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1.
Anti-NIT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY NIT1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY NIT1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 4/22/2019
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