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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NIT1 Antibody (clone 1C3)

NIT1 antibody LS-C197785 is an unconjugated mouse monoclonal antibody to human NIT1. Validated for ELISA, IF, IP and WB.
Catalog
Size
Price
LS-C197785-100
100 µg
$355
Description
NIT1 antibody LS-C197785 is an unconjugated mouse monoclonal antibody to human NIT1. Validated for ELISA, IF, IP and WB.
Target
Human NIT1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1C3]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
Immunogen
NIT1 antibody was raised against nIT1 (NP_005591.1, aa228-327 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human NIT1
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About NIT1
Q86X76 NM_005600 NP_005591.1

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Images

Immunofluorescence

Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.
Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.
Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.
Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to NIT1 on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.
Immunoprecipitation of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 1/24/2019
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