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Antibodies > NGF > LS-C483049
 
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Catalog Number Size Price
LS-C483049-100 100 µl $393 
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mm Hg with 300 ml of Davidson's modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions, using DAB chromogen. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mm Hg with 300 ml of Davidson's modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions, using DAB chromogen. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). WB on rat salivary gland lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mm Hg with 300 ml of Davidson's modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions, using DAB chromogen. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat salivary gland. The animal was perfused using Autoperfuser at a pressure of 130 mm Hg with 300 ml of Davidson's modified fixative (4% formaldehyde also works nicely) before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions, using DAB chromogen. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 110 mm Hg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
NGF Antibody - Rabbit antibody to NGF (130-180). WB on rat salivary gland lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.
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Polyclonal Rabbit anti‑Mouse NGF Antibody (aa130‑180, IHC, WB) LS‑C483049

Polyclonal Rabbit anti‑Mouse NGF Antibody (aa130‑180, IHC, WB) LS‑C483049

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Antibody:
NGF Rabbit anti-Mouse Polyclonal (aa130-180) Antibody
Application:
IHC, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C483049-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NGF Rabbit anti-Mouse Polyclonal (aa130-180) Antibody
Application:
IHC, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NGF antibody LS-C483049 is an unconjugated rabbit polyclonal antibody to NGF (aa130-180) from mouse. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Mouse NGF
Synonyms
NGF | Beta-nerve growth factor | ProNGF | Beta-NGF | HSAN5 | NGFB
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
Synthetic peptide from aa region of 130-180 of mouse NGF conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.
Epitope
aa130-180
Specificity
Specific for NGF.
Applications
  • IHC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized
Reconstitution
100 µl Sterile water
Storage
Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NGF
P01138 NM_002506 NP_002497.2

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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