Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the 25 kD B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) catalog no. LS-C60884
. Anti-NF-Y (13 subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (13 subunit specific). Minimal reaction was observed by immunoblot against the 35 kD A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 ul per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#LS-C60884
) Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y.