Research Areas
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Research Areas
Contact Us
NFYB Antibody (N‑Terminus) LS‑C18766
Note: This antibody replaces LS-C9330
NFYB antibody LS-C18766 is an unconjugated rabbit polyclonal antibody to human NFYB. Validated for ELISA, IP and WB.
100 µg

Popular NFYB Products

Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Transfected 293T Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: Western blot
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NFYB mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Immunofluorescence analysis of MCF7 cells.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation, ELISA, Gel shift

Product Description

NFYB antibody LS-C18766 is an unconjugated rabbit polyclonal antibody to human NFYB. Validated for ELISA, IP and WB.
About NFYB
NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. P25208 NM_006166 NP_006157.1

NFYB Antibody, CBF-A Antibody, CBF-B Antibody, HAP3 Antibody, NF-YB Antibody


Human NFYB
Human (tested or 100% immunogen sequence identity)
Ion exchange chromatography
  • Western blot (1:500)
  • Immunoprecipitation
NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the 25 kD B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti-NF-Y (13 subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (13 subunit specific). Minimal reaction was observed by immunoblot against the 35 kD A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 ul per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#LS-C60884) Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y.
0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only.

Publications (0)

Customer Reviews (0)

Requested From: United States
Date Requested: 9/20/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy