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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NFYB Antibody (N‑Terminus) IHC‑plus™ LS‑B718 Best Seller
Note: This antibody replaces LS-C18767
NFYB antibody LS-B718 is an unconjugated rabbit polyclonal antibody to human NFYB. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B718-50
50 µl (90 mg/ml)
$425

Popular NFYB Products

Transfected 293T Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: Western blot
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NFYB mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
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Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Antibody
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation, ELISA, Gel shift

Product Description

NFYB antibody LS-B718 is an unconjugated rabbit polyclonal antibody to human NFYB. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About NFYB
NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. P25208 NM_006166 NP_006157.1

NFYB Antibody, CBF-A Antibody, CBF-B Antibody, HAP3 Antibody, NF-YB Antibody


Specifications

Target
Human NFYB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
N-Terminus
Specificity
NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Usage
Immunohistochemistry: LS-B718 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B718 was determined to be 1:500.
Presentation
Serum, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.
Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.

Immunohistochemistry

Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.
Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.

Immunohistochemistry

Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.
Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.

Immunohistochemistry

Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.
Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.

Requested From: United States
Date Requested: 8/19/2018

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