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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NFYB Antibody (clone NFYBA7) LS‑C184290
NFYB antibody LS-C184290 is an unconjugated mouse monoclonal antibody to human NFYB. Validated for DB, ICC and WB.
Catalog
Size
Price
LS-C184290-50
50 µg (0.1 mg/ml)
$325

Popular NFYB Products

Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Transfected 293T Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: Western blot
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NFYB mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Immunofluorescence analysis of MCF7 cells.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Antibody
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation, ELISA, Gel shift

Product Description

NFYB Antibody (clone NFYBA7) for ICC, WB/Western LS-C184290

Specifications

Target
Human NFYB
Synonyms
NFYB, CBF-A, CBF-B, HAP3, NF-YB
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [NFYBA7]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC (2 - 100 µg/ml)
  • Western blot
  • Dot Blot
Immunogen
NFYB antibody was raised against recombinant Nuclear Transcription Factor Y, Beta (Nfyb).
Specificity
Human NFYB
Presentation
PBS, pH 7.4, 0.05% Sodium Azide, 1% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About NFYB
NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. P25208 NM_006166 NP_006157.1


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Images

Western blot

Western blot of immunized recombinant protein using NFYB antibody. Left: NFYB staining. Right: Coomassie Blue staining of immunized recombinant protein.
Western blot of immunized recombinant protein using NFYB antibody. Left: NFYB staining. Right: Coomassie Blue staining of immunized recombinant protein.

Western blot

Western blot of immunized recombinant protein using NFYB antibody. Left: NFYB staining. Right: Coomassie Blue staining of immunized recombinant protein.
Western blot of immunized recombinant protein using NFYB antibody. Left: NFYB staining. Right: Coomassie Blue staining of immunized recombinant protein.

Western blot

Western blot of immunized recombinant protein using NFYB antibody. Left: NFYB staining. Right: Coomassie Blue staining of immunized recombinant protein.
Western blot of immunized recombinant protein using NFYB antibody. Left: NFYB staining. Right: Coomassie Blue staining of immunized recombinant protein.

Western blot

Western blot of immunized recombinant protein using NFYB antibody. Left: NFYB staining. Right: Coomassie Blue staining of immunized recombinant protein.
Western blot of immunized recombinant protein using NFYB antibody. Left: NFYB staining. Right: Coomassie Blue staining of immunized recombinant protein.

Requested From: United States
Date Requested: 10/18/2018

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