Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the differentially spliced 35 and 42 kD forms of the A subunit of human and mouse NF-Y at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884
. No reaction was observed by immunoblot against the 25 kD B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 ul per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISA using (#LS-C60884
) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:10000 is suggested from this experiment. No reactivity was observed by ELISA against the B subunit of NF-Y.