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Catalog Number Size Price
LS-C18769-100 100 µg (0.6 mg/ml) $498 
NFYA Antibody - Anti-NF-Y(A subunit) Antibody - Western Blot. CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein were subjected to analysis by SDS-PAGE and western blot. After transfer, the membrane was probed using anti-NF-YA as the primary antibody at a 1:1000 dilution, followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L]. Signal was developed using an ECL kit followed by autoradiography using a 30 exposure. The antibody recognizes NF-YA as a doublet of ~42 kD in size.

Polyclonal Rabbit anti‑Human NFYA Antibody (N‑Terminus, IHC, WB) LS‑C18769

Polyclonal Rabbit anti‑Human NFYA Antibody (N‑Terminus, IHC, WB) LS‑C18769

Antibody:
NFYA Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18769-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NFYA Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NFYA antibody LS-C18769 is an unconjugated rabbit polyclonal antibody to human NFYA (N-Terminus). Validated for ELISA, IHC and WB.
Target
Human NFYA
Synonyms
NFYA | CBF-A | CBF-B | HAP2 | HAP2 CCAAT-binding protein | NF-YA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography
Modifications
Unmodified
Immunogen
Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
N-Terminus
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the differentially spliced 35 and 42 kD forms of the A subunit of human and mouse NF-Y at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. No reaction was observed by immunoblot against the 25 kD B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 ul per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISA using (#LS-C60884) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:10000 is suggested from this experiment. No reactivity was observed by ELISA against the B subunit of NF-Y.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -70°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFYA
P23511 NM_021705 NP_068351.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022