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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NFYA Antibody (N‑Terminus) IHC‑plus™ LS‑B131 Best Seller
Note: This antibody replaces LS-C18768
NFYA antibody LS-B131 is an unconjugated rabbit polyclonal antibody to human NFYA. Validated for ELISA, GS, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B131-50
50 µl
$425

Popular NFYA Products

Detection of Human and Mouse NF-YA by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-NF-YA used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
NFYA antibody LS-C31566 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Western blot of NF-YA antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation, ELISA

Product Description

NFYA antibody LS-B131 is an unconjugated rabbit polyclonal antibody to human NFYA. Validated for ELISA, GS, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About NFYA
NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. P23511 NM_021705 NP_068351.1

NFYA Antibody, CBF-A Antibody, CBF-B Antibody, HAP2 Antibody, HAP2 CCAAT-binding protein Antibody, NF-YA Antibody


Specifications

Target
Human NFYA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Sterile filtered
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:400)
  • Western blot (1:1000)
  • ELISA
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
N-Terminus
Specificity
NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Usage
Immunohistochemistry: LS-B131 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B131 was determined to be 1:400.
Presentation
Serum - liquid, 0.01% sodium azide.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-NFYA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B131 dilution 1:400.
Anti-NFYA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B131 dilution 1:400.

Immunohistochemistry

Anti-NFYA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B131 dilution 1:400.
Anti-NFYA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B131 dilution 1:400.

Immunohistochemistry

Anti-NFYA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B131 dilution 1:400.
Anti-NFYA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B131 dilution 1:400.

Immunohistochemistry

Anti-NFYA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B131 dilution 1:400.
Anti-NFYA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B131 dilution 1:400.

Requested From: United States
Date Requested: 8/21/2018

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