NFYA antibody LS-B131 is an unconjugated rabbit polyclonal antibody to human NFYA. Validated for ELISA, GS, IHC and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:400)
- Western blot (1:1000)
- Gel shift
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NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunohistochemistry: LS-B131 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B131 was determined to be 1:400.
Serum - liquid, 0.01% sodium azide.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.