Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
(tested or 100% immunogen sequence identity)
IHC - Paraffin (5 µg/ml)
Immunofluorescence (1:250 - 1:1000)
Immunoprecipitation (2 - 5 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Region between residue 320 and 333 of human nuclear transcription factor Y, alpha using the numbering given in entry NP_002496.1 (GeneID 4800).
Immunohistochemistry: Antigen retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Linitis Plastica Stomach Cancer, Non-Small Cell Lung Cancer, Prostate Carcinoma.
NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit.