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Catalog Number Size Price
LS-C482672-50 50 µl (1 mg/ml) $286 
LS-C482672-100 100 µl (1 mg/ml) $321 
LS-C482672-200 200 µl (1 mg/ml) $386 
NFS1 Antibody - Immunohistochemical analysis of NFS1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NFS1 Antibody - Immunofluorescent analysis of NFS1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
NFS1 Antibody - Western blot analysis of NFS1 expression in HepG2 (A); SKOV3 (B); mouse kidney (C); mouse heart (D); rat liver (E) whole cell lysates.
NFS1 Antibody - Immunohistochemical analysis of NFS1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NFS1 Antibody - Immunofluorescent analysis of NFS1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
NFS1 Antibody - Western blot analysis of NFS1 expression in HepG2 (A); SKOV3 (B); mouse kidney (C); mouse heart (D); rat liver (E) whole cell lysates.
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Polyclonal Rabbit anti‑Human NFS1 Antibody (IHC, IF, WB) LS‑C482672

Polyclonal Rabbit anti‑Human NFS1 Antibody (IHC, IF, WB) LS‑C482672

Antibody:
NFS1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C482672-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NFS1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NFS1 antibody LS-C482672 is an unconjugated rabbit polyclonal antibody to NFS1 from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human NFS1
Synonyms
NFS1 | Cysteine desulfurase | HUSSY-08 | IscS | NIFS
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant full length human NFS1.
Specificity
Recognizes endogenous levels of NFS1 protein.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFS1
Q9Y697 NM_021100 NP_066923.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/17/2024