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Catalog Number Size Price
LS-C358828-30 30 µl (1 mg/ml) $255 
LS-C358828-100 100 µl (1 mg/ml) $299 
LS-C358828-200 200 µl (1 mg/ml) $351 
NFKB1 / NF-Kappa-B Antibody - Immunohistochemical analysis of NF-kappaB p105 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NFKB1 / NF-Kappa-B Antibody - Immunofluorescent analysis of NF-kappaB p105 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of NF-kappaB p105 expression in HEK293T (A); HeLa (B); mouse brain (C); rat brain (D) whole cell lysates.
NFKB1 / NF-Kappa-B Antibody - Immunohistochemical analysis of NF-kappaB p105 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NFKB1 / NF-Kappa-B Antibody - Immunofluorescent analysis of NF-kappaB p105 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of NF-kappaB p105 expression in HEK293T (A); HeLa (B); mouse brain (C); rat brain (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (C‑Terminus, IHC, IF, WB) LS‑C358828

Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (C‑Terminus, IHC, IF, WB) LS‑C358828

Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C358828-30
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NF-Kappa-B antibody LS-C358828 is an unconjugated rabbit polyclonal antibody to NF-Kappa-B (NFKB1) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human NFKB1 / NF-Kappa-B
Synonyms
NFKB1 | Ebp- 1 | EBP-1 | NF-KappaB p50 | NFkappaB | NFKB-p50 | KBF1 | NF-kappa-B | NF-kappaB | NF-kB1 | NFKB-p105 | p105 | p50 | DNA binding factor KBF1 | DNA-binding factor KBF1 | NF-kappabeta
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human NF-kappaB p105.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of NF-kappaB p105 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKB1 / NF-Kappa-B
P19838 NM_003998 NP_003989.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024