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Catalog Number	Size	Price
LS-C661863-10	10 µg	$318
LS-C661863-100	100 µg	$470

NFATC1 / NFAT2 Antibody - Western blot - Anti-NFAT2 Picoband Antibody

NFATC1 / NFAT2 Antibody - Flow Cytometry analysis of MCF-7 cells using anti-NFATC1 antibody. Overlay histogram showing MCF-7 cells stained with anti-NFATC1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFATC1 Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

NFATC1 / NFAT2 Antibody - Flow Cytometry analysis of Jurkat cells using anti-NFATC1 antibody. Overlay histogram showing Jurkat cells stained with anti-NFATC1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFATC1 Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

NFATC1 / NFAT2 Antibody - Flow Cytometry analysis of Hela cells using anti-NFATC1 antibody. Overlay histogram showing Hela cells stained with anti-NFATC1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFATC1 Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

NFATC1 / NFAT2 Antibody - Flow Cytometry analysis of THP-1 cells using anti-NFATC1 antibody. Overlay histogram showing THP-1 cells stained with anti-NFATC1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFATC1 Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human NFATC1 / NFAT2 Antibody (IHC, WB) LS‑C661863

Name: Anti-NFATC1 / NFAT2 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C661863-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

NFATC1 / NFAT2 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, ICC, WB, Flo, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C661863-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

NFATC1 / NFAT2 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, ICC, WB, Flo, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

NFAT2 antibody LS-C661863 is an unconjugated rabbit polyclonal antibody to human NFAT2 (NFATC1). Validated for ELISA, Flow, ICC, IHC and WB.

Target

Human NFATC1 / NFAT2

Synonyms

See All NFATC1 / NFAT2 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived human NFAT2 recombinant protein (Position: Q589-K652).

Specificity

Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T- cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T- cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.

Applications

IHC
ICC
Western blot
Flow Cytometry
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About NFATC1 / NFAT2

Uniprot: O95644 NCBI: NM_006162 NP_006153.2