Human NES / Nestin
NES | Nestin | Nbla00170
(tested or 100% immunogen sequence identity)
Human, Rabbit, Macaque (at least 90% immunogen sequence identity)
Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and macaque (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from rabbit (94%), mouse (reactivity shown above), rat and hamster (88%), and bovine and dog (82%). Reactivity against homologues from other sources is not known.
- Western blot (1:500 - 1:3000)
- ELISA (1:20000 - 1:80000)
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Applications should be user optimized.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
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