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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C142113-100 100 µl (1.5 mg/ml) $365 
NES / Nestin Antibody - Anti-Nestin antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NES / Nestin Antibody - Visualization of nestin in PC-3 cells. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NES / Nestin Antibody - Nestin Antibody (10C2) - Detection of human nestin.  Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NES / Nestin Antibody - Flow Cytometry: Nestin Antibody (10C2) - FACS staining of NTERA-2 cells3 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NES / Nestin Antibody - Anti-Nestin antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NES / Nestin Antibody - Visualization of nestin in PC-3 cells. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NES / Nestin Antibody - Nestin Antibody (10C2) - Detection of human nestin.  Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NES / Nestin Antibody - Flow Cytometry: Nestin Antibody (10C2) - FACS staining of NTERA-2 cells3 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 10C2, DY488, aa1464‑1614, IHC, IF, WB) LS‑C142113

Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 10C2, DY488, aa1464‑1614, IHC, IF, WB) LS‑C142113

Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, ICC, IF, WB, Flo
Reactivity:
Human
Format:
DyLight 488, Unmodified
Other formats:
Price
Catalog Number
$365 
LS-C142113-100
100 µl
1.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human NES / Nestin
Application:
IHC, IHC-P, IHC-Fr, ICC, IF, WB, Flo
Reactivity:
Human
Format:
DyLight 488, Unmodified
Other formats:

Specifications

Description
Nestin antibody LS-C142113 is a DY488-conjugated mouse monoclonal antibody to human Nestin (NES) (aa1464-1614). Validated for Flow, ICC, IF, IHC and WB.
Target
Human NES / Nestin
Synonyms
NES | Nestin | Nbla00170
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat, Rabbit
Clonality
IgG1,k Monoclonal
Clone
10C2
Conjugations
DyLight 488. Also available conjugated with Biotin, DY650, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Immunofluorescence (1:1000 - 1:5000)
  • Western blot (1:1000 - 1:5000)
  • Flow Cytometry
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A fragment of the human nestin protein corresponding to residues 1464-1614.
Epitope
aa1464-1614
Specificity
Human nestin.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Borate, 0.05% Sodium Azide
Storage
Store at 4°C. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NES / Nestin
P48681 X65964 CAA46780.1

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Requested From: United States
Date Requested: 6/1/2020