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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NEK4 Antibody (aa74-101, PE) LS-C264201

NEK4 antibody LS-C264201 is a PE-conjugated rabbit polyclonal antibody to NEK4 from human and mouse. Validated for ELISA and WB.
Catalog
Size
Price
LS-C264201-200
200 µl
$740
Description
NEK4 antibody LS-C264201 is a PE-conjugated rabbit polyclonal antibody to NEK4 from human and mouse. Validated for ELISA and WB.
Target
Human NEK4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Phycoerythrin. Also available Unconjugated or conjugated with AP, APC, Biotin, FITC, HRP.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Epitope
aa74-101
Specificity
This Mouse Nek4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-101 amino acids from the N-terminal region of mouse Nek4.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, No preservatives added
Storage
Short term: store at 4°C. Long term: aliquot and store -20°C for up to 6 months. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only.
About NEK4
P51957 NM_003157 NP_003148.2

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Detection of Human NEK4 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NEK4 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Immunofluorescence staining of Hela cells at a dilution of 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
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Requested From: United States
Date Requested: 2/22/2019
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