Target
Human NEIL1
Synonyms
NEIL1 | DNA glycosylase/AP lyase Neil1 | Endonuclease VIII | Endonuclease 8-like 1 | Endonuclease VIII-like 1 | FPG1 | HFPG1 | Nei homolog 1 | NEI1 | Nei-like protein 1 | NEH1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Immunogen
Recombinant full length protein of human NEIL1
Specificity
Recognizes endogenous levels of NEIL1 protein.
Applications
- ICC
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
Usage
Western blot analysis of NEIL1 expression in A375 (A) whole cell lysates. Immunofluorescent analysis of NEIL1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Presentation
0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee