(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- Immunofluorescence (1:100)
- Western blot (1:1000)
NEFM / NF-M antibody was raised against recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli (1).
The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species. Recognizes intact NF-M strongly and specifically on Western Blots in all mammals and birds tested to date and also binds NF-M in cells in tissue culture and in sectioned material.
Suitable for use in Immunofluorescence, Western Blot. Immunofluorescence: 1:100. Western Blot: 1:1000.
PBS, pH 7.2, 0.1% Sodium Azide
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.