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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NEFH / NF‑H Antibody (clone NAP4) LS‑C2724
NF-H antibody LS-C2724 is an unconjugated mouse monoclonal antibody to NF-H (NEFH) from human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C2724-100
100 µl
$335
Description
NF-H antibody LS-C2724 is an unconjugated mouse monoclonal antibody to NF-H (NEFH) from human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Target
Pig NEFH / NF-H
Host
Mouse
Reactivity
Pig, Human, Mouse, Rat, Avian, Mammal (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [NAP4]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:5000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NEFH / NF-H antibody was raised against native pig spinal cord neurofilaments
Specificity
Specifically recognizes the phosphorylated variant of NF-H subunit (~180-220 kD), showing some weaker reactivity with phosphorylated forms of NF-M.
Presentation
pH 7.6, Sodium Phosphate, 0.25 M NaCl, 15 mg/ml BSA, 0.05% Sodium Azide, 0.01% Thimerosal
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About NEFH / NF-H
P12036 NM_021076 NP_066554.2

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Anti-NEFH antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Antibody
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Rat Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Images

Western blot

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H Antibody Neurofilament heavy chain Antibody (NF-H ) (lane 2).
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H Antibody Neurofilament heavy chain Antibody (NF-H ) (lane 2).

Western blot

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H Antibody Neurofilament heavy chain Antibody (NF-H ) (lane 2).
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H Antibody Neurofilament heavy chain Antibody (NF-H ) (lane 2).

Western blot

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H Antibody Neurofilament heavy chain Antibody (NF-H ) (lane 2).
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H Antibody Neurofilament heavy chain Antibody (NF-H ) (lane 2).

Western blot

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H Antibody Neurofilament heavy chain Antibody (NF-H ) (lane 2).
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H Antibody Neurofilament heavy chain Antibody (NF-H ) (lane 2).

Requested From: United States
Date Requested: 12/11/2018
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