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Antibodies > NEDD8 > LS-C661991
 
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Catalog Number Size Price
LS-C661991-10 10 µg $318 
LS-C661991-100 100 µg $470 
NEDD8 Antibody - NEDD8 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody
NEDD8 Antibody - Western blot analysis of NEDD8 using anti-NEDD8 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis cell lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: HELA whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD8 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NEDD8 at approximately 12KD. The expected band size for NEDD8 is at 9KD.
NEDD8 Antibody - Western blot - Anti-NEDD8 Picoband Antibody
NEDD8 Antibody - Flow Cytometry analysis of A431 cells using anti-NEDD8 antibody. Overlay histogram showing A431 cells stained with anti-NEDD8 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEDD8 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
NEDD8 Antibody - NEDD8 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- NEDD8 Antigen Affinity purified polyclonal antibody
NEDD8 Antibody - Western blot analysis of NEDD8 using anti-NEDD8 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis cell lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: HELA whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD8 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NEDD8 at approximately 12KD. The expected band size for NEDD8 is at 9KD.
NEDD8 Antibody - Western blot - Anti-NEDD8 Picoband Antibody
NEDD8 Antibody - Flow Cytometry analysis of A431 cells using anti-NEDD8 antibody. Overlay histogram showing A431 cells stained with anti-NEDD8 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEDD8 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human NEDD8 Antibody (IHC, WB) LS‑C661991

Polyclonal Rabbit anti‑Human NEDD8 Antibody (IHC, WB) LS‑C661991

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Antibody:
NEDD8 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661991-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NEDD8 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NEDD8 antibody LS-C661991 is an unconjugated rabbit polyclonal antibody to human NEDD8. Validated for IHC and WB.
Target
Human NEDD8
Synonyms
NEDD8 | NEDD-8 | Neddylin | Ubiquitin-like protein Nedd8
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NEDD8 (20-60 aa TDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYK), identical to the related mouse and rat sequences.
Specificity
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEDD8
Q15843 NM_006156 NP_006147.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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