Human NEDD1

Rabbit

Human (tested or 100% immunogen sequence identity)

Chimpanzee, Marmoset, Mouse, Rat, Bovine, Chicken, Turkey, Salmon, Macaque (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Affinity chromatography

Unmodified

Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.

This antibody reacts with endogenous Nedd1 protein. A BLAST analysis was used to suggest reactivity with Nedd1 from human, chimpanzee, macaque, marmoset, cattle, rat, and mouse based on a 100% homology with the immunizing sequence. Expect partial reactivity with Nedd1 from turkey, chicken, salmon, and Danio based on a 91% homology with the immunizing sequence. Cross-reactivity with Nedd1 from other sources has not been determined.

• Western blot (1:10000)
• ELISA (1:100000)

This antiserum has been tested for use in ELISA and western blotting using a recombinant truncated Nedd1 protein. Specific conditions for reactivity and detection of Nedd1 should be optimized by the end user. Expect a band approximately ~73 kD in size corresponding to Nedd1 by western blotting in the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q8NHV4 NCBI: Q8NHV4