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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C153764-100 100 µl (40 mg/ml) $425 
NEDD1 Antibody - Anti-NEDD1 in Western blot of Immunochemicals' Anti-NEDD1 Antibody shows detection of a 73 kDa band corresponding to endogenous NEDD1 in lysates of S phase HeLa cells silenced for either control Luciferase or NEDD1. In right lane (NEDD1i): lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-NEDD1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

NEDD1 Antibody LS‑C153764

NEDD1 Antibody LS‑C153764

Antibody:
Rabbit Polyclonal to Human NEDD1
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153764-100
100 µl
40 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NEDD1
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NEDD1 antibody LS-C153764 is an unconjugated rabbit polyclonal antibody to human NEDD1. Validated for ELISA and WB.
Target
Human NEDD1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:10000)
  • ELISA
Immunogen
Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.
Specificity
This antibody reacts with endogenous Nedd1 protein. A BLAST analysis was used to suggest reactivity with Nedd1 from human, chimpanzee, macaque, marmoset, cattle, rat, and mouse based on a 100% homology with the immunizing sequence. Expect partial reactivity with Nedd1 from turkey, chicken, salmon, and Danio based on a 91% homology with the immunizing sequence. Cross-reactivity with Nedd1 from other sources has not been determined.
Usage
This antiserum has been tested for use in ELISA and western blotting using a recombinant truncated Nedd1 protein. Specific conditions for reactivity and detection of Nedd1 should be optimized by the end user. Expect a band approximately ~73 kD in size corresponding to Nedd1 by western blotting in the appropriate cell lysate or extract.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEDD1
Q8NHV4 Q8NHV4

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Requested From: United States
Date Requested: 11/17/2019