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Catalog Number Size Price
LS-C745310-25 25 µl (0.82 mg/ml) $275 
NCOA3 / SRC-3 / AIB1 Antibody - Immunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
NCOA3 / SRC-3 / AIB1 Antibody - Immunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
NCOA3 / SRC-3 / AIB1 Antibody - Western blot using the affinity purified anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown).
NCOA3 / SRC-3 / AIB1 Antibody - Immunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
NCOA3 / SRC-3 / AIB1 Antibody - Immunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
NCOA3 / SRC-3 / AIB1 Antibody - Western blot using the affinity purified anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown).
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Polyclonal Rabbit anti‑Human NCOA3 / SRC‑3 / AIB1 Antibody (IHC, WB) LS‑C745310

Polyclonal Rabbit anti‑Human NCOA3 / SRC‑3 / AIB1 Antibody (IHC, WB) LS‑C745310

Antibody:
NCOA3 / SRC-3 / AIB1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745310-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
NCOA3 / SRC-3 / AIB1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AIB1 antibody LS-C745310 is an unconjugated rabbit polyclonal antibody to human AIB1 (NCOA3 / SRC-3). Validated for ELISA, IHC, IP and WB.
Target
Human NCOA3 / SRC-3 / AIB1
Synonyms
NCOA3 | ACTR | AIB1 | BHLHe42 | CAGH16 | CBP-interacting protein | CTG26 | AIB-1 | P/CIP | TNRC14 | TRAM-1 | Nuclear receptor coactivator 3 | PCIP | RAC-3 | SRC3 | TNRC16 | KAT13B | NCoA-3 | SRC-3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein.
Specificity
This antibody reacts with several isoforms of human NCOA3 protein (a, b, d, e and f). A BLAST analysis was used to suggest cross-reactivity with NCOA3 from human and macaque sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with NCOA3 from dog, bovine, mouse and rat sources based on partial sequence homology. Cross-reactivity with NCOA3 from other sources has not been determined.
Applications
  • IHC (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:100)
  • ELISA (1:175000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NCOA3 / SRC-3 / AIB1
Q9Y6Q9 NM_006534 NP_006525.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021